The explosive-degrading cytochrome P450 system is highly conserved among strains of Rhodococcus spp.
نویسندگان
چکیده
Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) is a widely used explosive and a serious environmental pollutant. Nineteen strains of Rhodococcus spp. capable of utilizing RDX as the sole nitrogen source have been isolated. The cytochrome P450 system XplA-XplB, which is responsible for RDX breakdown, is present in 18 of these strains.
منابع مشابه
Cloning, sequencing, and characterization of the hexahydro-1,3,5-Trinitro-1,3,5-triazine degradation gene cluster from Rhodococcus rhodochrous.
Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) is a high explosive which presents an environmental hazard as a major land and groundwater contaminant. Rhodococcus rhodochrous strain 11Y was isolated from explosive contaminated land and is capable of degrading RDX when provided as the sole source of nitrogen for growth. Products of RDX degradation in resting-cell incubations were analyzed and fou...
متن کاملA TaqMan polymerase chain reaction method for monitoring RDX-degrading bacteria based on the xplA functional gene.
Hexahydro-1,3,5-trinitro-1,3,5,-triazine (RDX) is a cyclic nitramine explosive that is a major component in many military high-explosive formulations. In this study, we developed a real-time TaqMan polymerase chain reaction (PCR) that targets the xplA functional gene involved in the breakdown/transformation of RDX. The xplA gene, described previously [Seth-Smith, H.M., Rosser, S.J., Basran, A.,...
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Repeated use of the explosive compound hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) on military land has resulted in significant soil and groundwater pollution. Rates of degradation of RDX in the environment are low, and accumulated RDX, which the U.S. Environmental Protection Agency has determined is a possible human carcinogen, is now threatening drinking water supplies. RDX-degrading microo...
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 74 14 شماره
صفحات -
تاریخ انتشار 2008